Generator

Part:BBa_K1976031:Design

Designed by: Sonja Elbrich, Claudia Kreher, Patrick Kunzmann, Nina Kuschik-Maczollek, Bianca Reisinger, Sabine Schäfer, Inka Schröter, Viktoria Schuster   Group: iGEM16_TU_Darmstadt   (2016-10-13)


Inducible generator for colicin E2 immunity protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 208
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Synthesis feasible by IDT, no amber stop codon for termination



Source

Escherichia coli


References